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Becton Dickinson
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Becton Dickinson
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Immunotec inc
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Miltenyi Biotec
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Becton Dickinson
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Thermo Fisher
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Image Search Results
Journal: Cancer immunology, immunotherapy : CII
Article Title: Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies
doi: 10.1007/s00262-012-1332-3
Figure Lengend Snippet: CD14+HLA-DR−/low MDSC are increased in patients with GI cancer compared to controls, and this increase is consistent across each method of processing. a Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). Relative frequency is shown in a while b illustrates the data calculated for estimated absolute number; c representative dot plots of CD14+HLA-DR−/low MDSC in fresh PBMC, cryopreserved PBMC or whole blood; d gating strategy; e gross morphology on cytospin
Article Snippet: For functional analysis, CD14 + HLA-DR −/low , CD14 + HLA-DR + , CD15 + CD14 − CD11b + CD33 + , and
Techniques: Flow Cytometry, Lysis
Journal: Cancer immunology, immunotherapy : CII
Article Title: Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies
doi: 10.1007/s00262-012-1332-3
Figure Lengend Snippet: CD15+CD14− cells but not CD15+CD14−CD11b+CD33+ are increased in patients with GI cancer compared to controls. Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). a Relative frequency CD15+CD14−;b estimated absolute number CD15+CD14 -; c relative frequency CD15+CD14−CD11b+CD33+; d estimated absolute number CD15+CD14−CD11b+CD33+; e representative dot plots of CD15+CD14−CD11b+CD33+ in fresh PBMC, cryopreserved PBMC or whole blood; f gross morphology on cytospin
Article Snippet: For functional analysis, CD14 + HLA-DR −/low , CD14 + HLA-DR + , CD15 + CD14 − CD11b + CD33 + , and
Techniques: Flow Cytometry, Lysis
Journal: Cancer immunology, immunotherapy : CII
Article Title: Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies
doi: 10.1007/s00262-012-1332-3
Figure Lengend Snippet: Analysis of CD14+HLA-DR−/low MDSC in patients with GI cancer. CD14+HLA-DR−/low, CD14+HLA-DR+, and CD15+CD14−CD11b+CD33+ cells were cocultured at different ratios with autologous anti-CD3/CD28−cells. Supernatant was taken from the cultures before addition of 3H-thymidine and measured for IFN-γ by ELISA. Results shown are representative of 3 independent experiments
Article Snippet: For functional analysis, CD14 + HLA-DR −/low , CD14 + HLA-DR + , CD15 + CD14 − CD11b + CD33 + , and
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Cancer immunology, immunotherapy : CII
Article Title: Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies
doi: 10.1007/s00262-012-1332-3
Figure Lengend Snippet: Correlations CD14+HLA-DR−/low MDSC across method of processing. a Fresh versus cryopreserved PBMC; b PBMC versus whole blood lysis; c whether absolute number or relative frequency calculated
Article Snippet: For functional analysis, CD14 + HLA-DR −/low , CD14 + HLA-DR + , CD15 + CD14 − CD11b + CD33 + , and
Techniques: Lysis
Journal: Cancer immunology, immunotherapy : CII
Article Title: Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies
doi: 10.1007/s00262-012-1332-3
Figure Lengend Snippet: Analysis of CD14+HLA-DR−/low MDSC in patients with GI cancer. CD14+HLA-DR−/low, CD14+HLA-DR+, and CD15+CD14−CD11b+CD33+ cells were cocultured at different ratios with autologous anti-CD3/CD28−cells. Supernatant was taken from the cultures before addition of 3H-thymidine and measured for IFN-γ by ELISA. Results shown are representative of 3 independent experiments
Article Snippet: For functional analysis, CD14 + HLA-DR −/low , CD14 + HLA-DR + , CD15 + CD14 − CD11b + CD33 + , and CD15 + CD14 − CD11b + CD33 − populations were incubated at different ratios with
Techniques: Enzyme-linked Immunosorbent Assay